Isolation of Type-specific Polysaccharide Antigen
نویسنده
چکیده
Lancefield et al. (1) have clearly demonstrated that the capsular polysaccharides of group B streptococci are antigenic and that the antibodies are protective in mice except with type III, where no mouse-virulent strain is available. However, a chicken embryo model has been established for type III and the antibody found to be specifically protective (2). Recently, emphasis has been centered on the isolation and characterization of the capsular antigens from various types (3-8). However, inasmuch as the capsule is very tightly (probably covalently) bound to the cell wall, a major problem is to find an extraction method that will give satisfactory yield. Conventional HC1 extraction results in partial destruction of the polysaccharide antigens with the loss of the sialic acid determinant (6-8). Current investigations have concentrated on obtaining larger size capsular polysaccharides that are antigenically intact. Neutral buffer extractions have been used successfully to obtain such molecules from type Ia and type III (3, 5). However, the yield is low, which hinders structural studies as well as more extensive clinical and immunological investigations. In this report, we describe the isolation of type Ib polysaccharide from whole cells by digestion with muralytic enzymes prepared from Streptomyces albus. The composition and immunological properties of the purified type Ib antigen are also discussed.
منابع مشابه
Isolation of type-specific polysaccharide antigen from group B type Ib streptococci
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تاریخ انتشار 2003